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Abstract Background:Protein presence information is an essential component of biological pathway identification. Presence of certain enzymes in an organism points towards the metabolic pathways that occur within it, whereas the absence of these enzymes indicates either the existence of alternative pathways or a lack of these pathways altogether. The same inference applies to regulatory pathways such as gene regulation and signal transduction. Protein presence information therefore forms the basis for biological pathway studies, and patterns in presence-absence across multiple organisms allow for comparative pathway analyses. Results:Here we present ProTaxoVis, a novel bioinformatic tool that extracts protein presence information from database queries and maps it to a taxonomic tree or heatmap. ProTaxoVis generates a large-scale overview of presence patterns in taxonomic clades of interest. This overview reveals protein distribution patterns, and this can be used to deduce pathway evolution or to probe other biological questions. ProTaxoVis combines and filters sequence query results to extract information on the distribution of proteins and translates this information into two types of visual outputs: taxonomic trees and heatmaps. The trees supplement their topology with scaled pie-chart representations per node of the presence of target proteins and combinations of these proteins, such that patterns in taxonomic groups can easily be identified. The heatmap visualisation shows presence and conservation of these proteins for a user-determined set of species, allowing for a more detailed view over a larger group of proteins as compared to the trees. ProTaxoVis also allows for visual quality checks of hits based on a coverage plot and a length histogram, which can be used to determine e-value and minimum protein length cutoffs. Tabular output of resulting data from the query, combined, and heatmap building step are saved and easily accessible for further analyses. Conclusions:We evaluate our tool with the phosphoribosyltransferases, a transferase enzyme family with notable distribution patterns amongst organisms of varying complexities and across Eukaryota, Bacteria, and Archaea. ProTaxoVis is open-source and available at:https://github.com/MolecularBioinformatics/ProTaxoVis. 

Abstract Model projections predict increasing temperatures and precipitation change in many locations in the Central United States. To provide perspective on what these trends might bring relative to what has already happened, we compared historical temperature and precipitation change with what models from the Coupled Model Intercomparison Project (CMIP6) predict. The analysis focuses on regions represented by five long‐term agroecosystem research sites along a latitudinal transect from Michigan to Iowa, Missouri, Oklahoma, and Mississippi. We analyzed trends in long‐term records (≥50 years) of precipitation and temperature data at annual and monthly scales using indicators that characterize extreme and average temperature and rainfall amounts. Results show that temperatures have changed from 1900 to 2020, more for minimum (0.1°C–0.3°C decade⁻¹) than maximum (−0.1°C–0.2°C decade⁻¹), more for winter (−0.1°C–0.3°C decade⁻¹) than summer (−0.1°C–0.1°C decade⁻¹), and more often in the north than in the south. Except in Mississippi, annual precipitation has increased at rates of 25 mm decade⁻¹or greater over 1950–2020, but monthly trends were inconsistent. Projected trends suggest continued temperature increases, highlighting the urgent need for research on management systems that are resilient to such increases.